您好,欢迎来到上海分类信息网
免费发信息

lovebet爱博滚球平台-lovebet爱博app-爱博love体育

2019-11-9 0:15:53发布3次查看ip:发布人:
产品编号 30-3242r
英文名称 phospho-c-kit(tyr721)
中文名称 磷酸化原癌基因c-kit抗体
别    名 c-kit (phospho y721); c-kit (phospho tyr721); p-c-kit (tyr 721); c kit; c-kit; cd 117; cd117; cd117 antigen; kit; kit_human; mast/stem cell growth factor receptor; mast/stem cell growth factor receptor kit; p145 c-kit; pbt; piebald trait protein; proto oncogene c kit; proto oncogene tyrosine protein kinase kit; proto-oncogene c-kit; scf receptor; scfr; soluble kit variant 1; stem cell factor receptor; tyrosine protein kinase kit; tyrosine-protein kinase kit; v kit hardy zuckerman 4 feline sarcoma viral oncogene homolog; v kit hardy zuckerman 4 feline sarcoma viral oncogene like protein; v-kit hardy-zuckerman 4 feline sarcoma viral oncogene homolog.
说 明 书 0.1ml  
产品类型 磷酸化抗体 
研究领域 肿瘤  免疫学  信号转导  转录调节因子  激酶和磷酸酶  
抗体来源 rabbit
克隆类型 polyclonal
交叉反应 human, mouse, rat, dog, pig, cow, horse, 
产品应用 wb=1:100-500 elisa=1:500-1000 ihc-p=1:100-500 ihc-f=1:100-500 if=1:100-500 (石蜡切片需做抗原修复) 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 145kda
细胞定位 细胞浆 细胞膜 
性    状 lyophilized or liquid
浓    度 1mg/1ml
免 疫 原 klh conjugated synthesised phosphopeptide derived from human c-kit around the phosphorylation site of tyr721
亚    型 igg
纯化方法 affinity purified by protein a
储 存 液 0.01m tbs(ph7.4) with 1% bsa, 0.03% proclin300 and 50% glycerol.
保存条件 store at -20 °c for one year. avoid repeated freeze/thaw cycles. the lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°c. when reconstituted in sterile ph 7.4 0.01m pbs or diluent of antibody the antibody is stable for at least two weeks at 2-4 °c.
pubmed pubmed
产品介绍 background:
c-kit is a transmembrane tyrosine kinase encoded by the ckit proto oncogene. c-kit acts to regulate a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. ligand binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. mutations in c-kit have been found to be important for tumor growth and progression in a variety of cancers including mast cell diseases, gastrointestinal stromal tumor, acute myeloid leukemia, ewing sarcoma and lung cancer. phosphorylation at tyrosine 721 of c-kit allows binding and activation of pi3 kinase.
function:
tyrosine-protein kinase that acts as cell-surface receptor for the cytokine kitlg/scf and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. in response to kitlg/scf binding, kit can activate several signaling pathways. phosphorylates pik3r1, plcg1, sh2b2/aps and cbl. activates the akt1 signaling pathway by phosphorylation of pik3r1, the regulatory subunit of phosphatidylinositol 3-kinase. activated kit also transmits signals via grb2 and activation of ras, raf1 and the map kinases mapk1/erk2 and/or mapk3/erk1. promotes activation of stat family members stat1, stat3, stat5a and stat5b. activation of plcg1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate.
subunit:
monomer in the absence of bound kitlg/scf. homodimer in the presence of bound kitlg/scf, forming a heterotetramer with two kitlg/scf molecules. interacts (via phosphorylated tyrosine residues) with the adapter proteins grb2 and grb7 (via sh2 domain), and sh2b2/aps. interacts (via c-terminus) with mpdz (via the tenth pdz domain). interacts (via phosphorylated tyrosine residues) with pik3r1 and pik3 catalytic subunit. interacts (via phosphorylated tyrosine) with crk (isoform crk-ii), fyn, shc1 and matk/chk (via sh2 domain). interacts with lyn and fes/fps. interacts (via phosphorylated tyrosine residues) with the protein phosphatases ptpn6/shp-1 (via sh2 domain), ptpn11/shp-2 (via sh2 domain) and ptpru. interacts with plcg1. interacts with dok1 and tec. subcellular location : isoform 1: cell membrane; single-pass type i membrane protein. isoform 2: cell membrane; single-pass type i membrane protein. isoform 3: cytoplasm. note=detected in the cytoplasm of spermatozoa, especially in the equatorial and subacrosomal region of the sperm head.
subcellular location:
isoform 1: cell membrane; single-pass type i membrane protein. isoform 2: cell membrane; single-pass type i membrane protein. isoform 3: cytoplasm. note=detected in the cytoplasm of spermatozoa, especially in the equatorial and subacrosomal region of the sperm head.
tissue specificity:
isoform 1 and isoform 2 are detected in spermatogonia and leydig cells. isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). widely expressed. detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells.
post-translational modifications:
ubiquitinated by socs6. kit is rapidly ubiquitinated after autophosphorylation induced by kitlg/scf binding, leading to internalization and degradation. 
autophosphorylated on tyrosine residues. kitlg/scf binding enhances autophosphorylation. isoform 1 shows low levels of tyrosine phosphorylation in the absence of added kitlg/scf (in vitro). kinase activity is down-regulated by phosphorylation on serine residues by protein kinase c family members. phosphorylation at tyr-568 is required for interaction with ptpn11/shp-2, crk (isoform crk-ii) and members of the src tyrosine-protein kinase family. phosphorylation at tyr-570 is required for interaction with ptpn6/shp-1. phosphorylation at tyr-703, tyr-823 and tyr-936 is important for interaction with grb2. phosphorylation at tyr-721 is important for interaction with pik3r1. phosphorylation at tyr-823 and tyr-936 is important for interaction with grb7.
disease:
defects in kit are a cause of piebald trait (pbt) [mim:172800]. pbt is an autosomal dominant genetic developmental abnormality of pigmentation characterized by congenital patches of white skin and hair that lack melanocytes. 
defects in kit are a cause of gastrointestinal stromal tumor (gist) [mim:606764]. 
defects in kit have been associated with testicular germ cell tumor (tgct) [mim:273300]. a common solid malignancy in males. germ cell tumors of the testis constitute 95% of all testicular neoplasms. 
defects in kit are a cause of acute myelogenous leukemia (aml) [mim:601626]. aml is a malignant disease in which hematopoietic precursors are arrested in an early stage of development. note=somatic mutations that lead to constitutive activation of kit are detected in aml patients. these mutations fall into two classes, the most common being in-frame internal tandem duplications of variable length in the juxtamembrane region that disrupt the normal regulation of the kinase activity. likewise, point mutations in the kinase domain can result in a constitutively activated kinase.
similarity:
belongs to the protein kinase superfamily. tyr protein kinase family. csf-1/pdgf receptor subfamily.
contains 5 ig-like c2-type (immunoglobulin-like) domains.
contains 1 protein kinase domain.
gene id:
3815
database links:
entrez gene: 3815 human
entrez gene: 16590 mouse
entrez gene: 64030 rat
omim: 164920 human
swissprot: p10721 human
swissprot: p05532 mouse
unigene: 479754 human
unigene: 247073 mouse
unigene: 54004 rat
important note:
this product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品图片 tissue/cell: human lung carcinoma; 4% paraformaldehyde-fixed and paraffin-embedded; 
antigen retrieval: citrate buffer ( 0.01m, ph 6.0 ), boiling bathing for 15min; block endogenous peroxidase by 3% hydrogen peroxide for 30min; blocking buffer (normal goat serum,c-0005) at 37℃ for 20 min; 
incubation: anti-phospho-c-kit (tyr721) polyclonal antibody, unconjugated(bs-3242r) 1:200, overnight at 4°c, followed by conjugation to the secondary antibody(sp-0023) and dab(c-0010) staining

上海真奥生物科技有限公司

13761041385
中国 上海
该用户其它信息

VIP推荐

  • 欢迎访问】上海好太太晾衣架~021-36377161官方网站上海各点lovebet爱博滚球平台维修电话欢迎您!
  • 欢迎进入【太仓开利空调各点】lovebet爱博滚球平台网站咨询电话
  • 2018年北京举办周嘉荣宗筋疗法培训班
  • 菠萝格和柳桉木的区别
  • 如何消除防水涂料施工中的针孔?
  • 怎样识别防腐木的质量是好是坏
  • 欢迎进入-)南京约克中央空调各点lovebet爱博滚球平台网站"咨询电话
  • 欢迎访问」贵阳市美菱冰箱官方网站全国各点lovebet爱博滚球平台咨询电话
  • {欢迎访问】~武汉荣事达冰箱官方网站全国各点lovebet爱博滚球平台咨询电话欢迎您
  • 欢迎来电】奥普浴霸维修-上海奥普浴霸热风换气维修
  • 欢迎进入+)金华海尔电器各点lovebet爱博滚球平台网站"咨询电话
  • 欢迎访问{北京裕豪开水器网站&lovebet爱博滚球平台中心}咨询电话
  • 欢迎访问】昆明海尔冰箱、洗衣机售后维修【网站】昆明各区售后维修服务中心
  • 郫县博世煤气灶lovebet爱博滚球平台>欢迎光临 -<!>官方网站
  • 欢迎访问宁波奉化区(欧琳燃气灶官方网站)lovebet爱博滚球平台咨询电话
  • 欢迎访问=成都四季沐歌太阳能全国各中心lovebet爱博滚球平台维修咨询+-电话
  • 欢迎访问~~青岛『小鸭太阳能』官方网站全市各区点售后维修服务咨询电话您
  • 上海到全国回程车调度、价格优惠
  • 欢迎访问{太原长虹冰箱#官方网站}太原各中心售后维修服务咨询#
  • 欢迎访问」慈溪三星洗衣机网站全国各点lovebet爱博滚球平台咨询电话~中心
  • 58便民服务【容声冰箱】太原各点售后电话公司维修费用】客服咨询中心
  • 欢迎访问】昆山华帝油烟机官方网点lovebet爱博滚球平台电话-中心
  • 欢迎访问】昆山光芒太阳能官方网站昆山各点lovebet爱博滚球平台-中心
  • 欢迎访问】成都前锋热水器官方网站成都各点lovebet爱博滚球平台-中心
  • 欢迎访问@长沙华天成空气能公司网站各中心lovebet爱博滚球平台维修咨询电话
  • 欢迎访问】湘潭小天鹅洗衣机官方网站湘潭各点lovebet爱博滚球平台-中心
  • 『欢迎访问』咸宁华扬太阳能』官方网站全国各区域lovebet爱博滚球平台维修咨询电话~欢迎您】
  • 『欢迎访问』太原皇明太阳能官方网站全国各中心lovebet爱博滚球平台维修咨询电话
  • %欢迎访问%——南通西门子冰箱网站(全市)各中心lovebet爱博滚球平台维修咨询电话
  • 成都劳特斯中央空调维修中心,专修大型中央空调,检修保养
  • {欢迎访问-}「中山三星电视机官方网站」各点lovebet爱博滚球平台维修中心电话
  • 『欢迎访问』上海德意燃气灶官方网站全国各中心lovebet爱博滚球平台维修咨询电话欢迎您
  • 『欢迎访问』上海伊莱克斯煤气灶官方网站全国各中心lovebet爱博滚球平台维修咨询电话欢迎您
  • 〖欢迎访问〗成都海尔官方网站成都各点lovebet爱博滚球平台>中心
  • 欢迎访问】太仓夏普电视机官方网站太仓各点lovebet爱博滚球平台-中心
  • 欢迎访问】郑州西门子洗衣机官方网站郑州各点lovebet爱博滚球平台-中心
  • 重庆到毕节物流有限公司欢迎您√
  • 『欢迎访问』上海格力空调官方网站全国各中心lovebet爱博滚球平台维修咨询电话欢迎您
  • 欢迎访问」沙坪坝区西门子洗衣机官方网站全市各点lovebet爱博滚球平台咨询电话-中心
  • 欢迎访问武汉市蔡甸区格力空调官方网站全国各站点lovebet爱博滚球平台维修咨询电话欢迎您
  • 昆明到海口托运小轿车有限公司{欢迎您}
  • 欢迎访问=上饶万和油烟机售后维修电话=诚信服务((进))万家
  • 欢迎访问」常州三菱电机中央空调官方网站全国各点lovebet爱博滚球平台咨询电话-中心
  • 欢迎访问#嘉兴奥克斯空调各维修点lovebet爱博滚球平台网站电话?(本公司承诺:随叫随到,就地服务,决不会乱收费)
  • 重庆到辛集返空车回程车货车信息部
  • 南昌到北京物流公司欢迎您18079178550
  • 欢迎访问=蚌埠格兰仕空调维修加氟移机网站lovebet爱博滚球平台涧西区维修咨询电话
  • 上海分类信息网-上海免费发布信息-上海新闻网